Fig. 2

Basic properties of hippocampal CA1 pyramidal cells remain stable in lipopolysaccharide (LPS) treated rats. a Schematic diagram illustrates the experimental sequence used. LPS injection (6 mg/kg i.p.) occurred 6 h before slicing. Apamin (100 nM), physostigmine (10 μM, physo) and TBPB (3 μM) were applied to brain slices in vitro. b Representative current clamp recordings showing action potentials in response to a depolarizing current injection step (+300 pA, 1 s). Scale bars: 50 mV, 500 pA, 500 ms. c, d Representative EPSC responses to a paired-pulse stimulation protocol with 50 ms (c) and 100 ms (d) inter-pulse intervals (scale bars: 50 pA, 100 ms). e, f Summary data from all cells showing the number of APs during a 1 s 300 pA depolarizing current injection (e) and resting membrane potential f. ** p < 0.01, ANOVA followed by Dunnett’s multiple comparisons test. g, h Scatter plots of the paired pulse ratios (amplitude EPSC2/amplitude EPSC1) measured from all cells using 50 ms (g) and 100 ms (h) inter-pulse intervals. Bars represent mean values (e, f, g, h). Control: n = 15 cells; LPS: n = 10 cells; control + apamin: n = 6 cells; LPS + apamin: n = 8 cells; control + physo: n = 6 cells; LPS + physo: n = 5 cells; control + TBPB: n = 7 cells; LPS + TBPB: n = 7 cells. TBPB: [1-(1′-2-methylbenzyl)-1,4′-bipiperidin-4-yl)-1H-benzo[d]imidazol-2(3H)-one]