Fig. 3

Macrophage infiltration in severely affected patients. a Immunofluorescent analysis of CD68 expression in biopsies from an adult control (left) or a severely affected adult onset Pompe patient (right). White arrows point to CD68-positive cells located between fibers, yellow arrowhead to CD68-positive cell invading a myofiber. b Detail of a necrotic fiber from the patient shown in Fig. 3a (white arrow) with CD68-positive cells around and inside the fiber. c GMA-fixed HE stained biopsy from a mildly affected Pompe patient