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Table 3 Stereological parameters and procedures applied to estimate neuronal soma volume

From: Significant neuronal soma volume deficit in the limbic system in subjects with 15q11.2-q13 duplications

Structures and their subdivisions Number of sections examined (per case) Obj. Grid size (μm) Test area size (μm) Mean number ± SD CEa
      Virtual counting spaces (per case) Neurons measured (per case)  
Amygdala 5 40× 2,000 × 2,000 60 × 60 133 ± 32 170 ± 5 0.01
Thalamus 6 40× 2,000 × 2,000 60 × 60 286 ± 82 219 ± 15 0.01
Entorhinal cortex (EC): Isl 3 100× 400 × 400 50 × 50 69 ± 27 107 ± 4 0.004
EC: L III 3 100× 800 × 800 50 × 50 57 ± 17 106 ± 4 0.004
Ammon’s horn: CA1 3 100× 1,000 × 1,000 50 × 50 41 ± 13 107 ± 4 0.004
CA2 3 100× 400 × 400 50 × 50 30 ± 10 106 ± 3 0.003
CA3 3 100× 400 × 400 50 × 50 44 ± 17 105 ± 3 0.004
CA4 3 100× 600 × 600 50 × 50 37 ± 15 105 ± 4 0.004
Caudate nucleus 4 63× 1,000 × 1,000 80 × 80 197 ± 70 340 ± 78 0.001
Putamen 4 63× 1,000 x 1,000 80 × 80 162 ± 75 329 ± 103 0.001
Globus pallidus 6 63× 500 x 500 60 × 60 663 ± 263 190 ± 97 0.001
Nucleus accumbens 6 63× 500 × 500 80 × 80 306 ± 100 447 ± 115 0.003
Magnocellular LGN 4 40× 500 × 500 100 × 100 211 ± 49 353 ± 33 0.001
Parvocellular LGN 4 40× 500 × 500 100 × 100 211 ± 49 353 ± 33 0.001
Claustrum 5 40× 600 × 600 60 × 60 417 ± 287 276 ± 44 0.01
Substantia nigra 8 63× 300 × 300 80 × 80 392 ± 104 231 ± 97 0.003
Magnocellular basal complex Ch1 4 63x 300 x 300 80 x 80 41 ± 12 87 ± 26 0.007
Ch2 4 63× 300 × 300 80 × 80 64 ± 16 143 ± 35 0.003
Ch3 4 63× 300 x 300 80 × 80 90 ± 39 189 ± 47 0.003
Ch4 4 63× 300 × 300 80 × 80 133 ± 49 240 ± 42 0.002
Purkinje cells 5 40× 1,800 × 1,800 180 × 180 1,118 ± 209 449 ± 28 0.001
Dentate nucleus 4 40× 1,000 × 1,000 180 × 180 178 ± 39 425 ± 28 0.002
Inferior olive 6 40× 600 × 600 80 × 80 110 ± 28 129 ± 23 0.003
  1. Obj. objective lens, CE average predicted coefficient of error of the measured neurons (Scheaffer), LGN lateral geniculate nucleus