Significant neuronal soma volume deficit in the limbic system in subjects with 15q11.2-q13 duplications

Wegiel, Jerzy; Flory, Michael; Schanen, N. Carolyn; Cook, Edwin H.; Nowicki, Krzysztof; Kuchna, Izabela; Imaki, Humi; Ma, Shuang Yong; Wegiel, Jarek; London, Eric; Casanova, Manuel F.; Wisniewski, Thomas; Brown, W. Ted

doi:10.1186/s40478-015-0241-z

Acta Neuropathologica Communications

Table 3 Stereological parameters and procedures applied to estimate neuronal soma volume

From: Significant neuronal soma volume deficit in the limbic system in subjects with 15q11.2-q13 duplications

Structures and their subdivisions	Number of sections examined (per case)	Obj.	Grid size (μm)	Test area size (μm)	Mean number ± SD		CE^a
					Virtual counting spaces (per case)	Neurons measured (per case)
Amygdala	5	40×	2,000 × 2,000	60 × 60	133 ± 32	170 ± 5	0.01
Thalamus	6	40×	2,000 × 2,000	60 × 60	286 ± 82	219 ± 15	0.01
Entorhinal cortex (EC): Isl	3	100×	400 × 400	50 × 50	69 ± 27	107 ± 4	0.004
EC: L III	3	100×	800 × 800	50 × 50	57 ± 17	106 ± 4	0.004
Ammon’s horn: CA1	3	100×	1,000 × 1,000	50 × 50	41 ± 13	107 ± 4	0.004
CA2	3	100×	400 × 400	50 × 50	30 ± 10	106 ± 3	0.003
CA3	3	100×	400 × 400	50 × 50	44 ± 17	105 ± 3	0.004
CA4	3	100×	600 × 600	50 × 50	37 ± 15	105 ± 4	0.004
Caudate nucleus	4	63×	1,000 × 1,000	80 × 80	197 ± 70	340 ± 78	0.001
Putamen	4	63×	1,000 x 1,000	80 × 80	162 ± 75	329 ± 103	0.001
Globus pallidus	6	63×	500 x 500	60 × 60	663 ± 263	190 ± 97	0.001
Nucleus accumbens	6	63×	500 × 500	80 × 80	306 ± 100	447 ± 115	0.003
Magnocellular LGN	4	40×	500 × 500	100 × 100	211 ± 49	353 ± 33	0.001
Parvocellular LGN	4	40×	500 × 500	100 × 100	211 ± 49	353 ± 33	0.001
Claustrum	5	40×	600 × 600	60 × 60	417 ± 287	276 ± 44	0.01
Substantia nigra	8	63×	300 × 300	80 × 80	392 ± 104	231 ± 97	0.003
Magnocellular basal complex Ch1	4	63x	300 x 300	80 x 80	41 ± 12	87 ± 26	0.007
Ch2	4	63×	300 × 300	80 × 80	64 ± 16	143 ± 35	0.003
Ch3	4	63×	300 x 300	80 × 80	90 ± 39	189 ± 47	0.003
Ch4	4	63×	300 × 300	80 × 80	133 ± 49	240 ± 42	0.002
Purkinje cells	5	40×	1,800 × 1,800	180 × 180	1,118 ± 209	449 ± 28	0.001
Dentate nucleus	4	40×	1,000 × 1,000	180 × 180	178 ± 39	425 ± 28	0.002
Inferior olive	6	40×	600 × 600	80 × 80	110 ± 28	129 ± 23	0.003

Obj. objective lens, CE average predicted coefficient of error of the measured neurons (Scheaffer), LGN lateral geniculate nucleus

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ISSN: 2051-5960

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