Fig. 6

Soma plasmalemma Aqp4 and Aqp4 abundance after MCAO. a-f micrographs of rat brain tissue immunolabeled for Aqp4 (white) and co-labeled for GFAP (red) in the control (CTR) cortex (a), the control subcortical white matter (d), the cortical penumbra at 10 and 48 hours reperfusion (b, c), or the subcortical white matter ipsilateral to MCAO at 10 and 48 hours reperfusion (e, f) showing low Aqp4 immunoreactivity at the astrocyte soma plasmalemma in the cortical penumbra at 48 hours reperfusion and increased Aqp4 at the astrocyte soma plasmalemma in the subcortical white matter at 48 hours reperfusion. g Aqp4 immunoblot of control tissue and cortical penumbra and subcortical white matter following 2 hour ischemia and 48 hours of reperfusion; 48 h = tissue obtained from rats submitted to 120 minute MCAO and 48 hours of reperfusion. h, i) visualization of quantification of immunoblot in G for Aqp4 monomer isoforms (h) and Aqp4 dimer isoforms (i); the ordinate reflects Aqp4 isoform band optical density normalized to Hsc-70 band optical density and then normalized to control tissue baseline; C = control tissue; n = 4 rats per group; * p < 0.05 comparison to the control baseline, depicted as dotted horizontal line