Fig. 3

A rat model of ischemic stroke. a image of triphenyl tetrazolium chloride (TTC) stained rat brain coronal section, taken through the MCA territory after 2 hour ischemia MCAO with 24 hours of reperfusion showing the extent of the ischemic lesion; scale bar 1 mm. b montage of micrographs of rat brain tissue after 2 hour ischemia MCAO and 24 hours of reperfusion processed for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (white) showing the spread of cell death that ends at penumbral tissue located at the ACA-MCA watershed region; field of view corresponds to the dashed region in A. c montage of micrographs shown in B merged with DAPI channel (blue) showing the location of the cortical penumbra ROI (ROI 1), the ischemic core ROI (ROI 2), and the subcortical white matter ROI (ROI 3) used for analysis of immunohistochemistry; field of view corresponds to the dashed region in A. d montage of micrographs of a coronal section after 2 hour ischemia MCAO and 24 hours of reperfusion processed for silver infarct staining (SIS) showing reduced staining in the ischemic grey matter and ischemic white matter; scale bar 1 mm. e visualization of analysis of silver infarct staining in cortex and white matter; Con. = contralateral hemisphere; Ips. = ipsilateral hemisphere; n = 3 rats per group; * p < 0.05 in comparison to the contralateral baseline, depicted as dotted horizontal line