Fig. 2

Aqp4 in ischemic human subcortical white matter. a, b montages of micrographs of tissue from control (CTR) human subcortical white matter (a) or ischemic human subcortical white matter from patients with ischemic stroke (b) immunolabeled for Aqp4 (white), showing minimal Aqp4 expression in control tissue with scarce Aqp4 positive cells (filled arrowheads) and increased Aqp4 immunoreactivity in ischemic white matter in ramified cells (filled arrowheads) and surrounding vessels (empty arrowhead); scale bars 200 μm. (c-g) micrographs of control white matter (c, e) or ischemic white matter (d, f, g) immunolabeled for Aqp4 (white) and co-labeled for CD31 (red) (c, d) or S100 (red) (e, f, g) showing increased perivascular Aqp4 in the ischemic white matter (d), increased Aqp4 in the somata and processes of white matter astrocytes in the ischemic white matter (f), and rare Aqp4 negative astrocytes in the ischemic white matter (filled arrowhead) (g); micrographs in C and E depict the merged fluorescent channels for Aqp4 and either CD31 or S100; micrographs in D, F, and G individually depict the Aqp4 channel, the CD31 or S100 channel, and the merged fluorescent image; scale bars 20 μm