Fig. 1
APP expression and Aβ oligomer accumulation in APP-transfected neurons. Mouse primary neurons were transfected with two plasmid vectors to simultaneously express APP and GFP. a Cells were stained with DAPI and human APP-specific 6E10 antibody (red). All GFP-positive neurons expressed human APP. Scale bar, 200 μm. b Cell homogenates were subjected to Western blot with 6E10 and anti-actin antibodies. c No significant difference in the levels of human APP expression between APPWT- and APPOSK-transfectants. AU, arbitrary unit. d Cells were stained with 6E10 (red) or Aβ oligomer-specific 11A1 antibody (red). Only APPOSK-expressing neurons accumulated abundant iAβOs. Scale bar, 30 μm