Fig. 3
Generation of disease-relevant iPSCs carrying all mutant mitochondrial tRNATrp. a Representative images of the established iPSC lines; OCT4 (red), NANOG (red), TRA-1-60 (green), TRA-1-81 (green). Arrowheads in electropherograms indicate m.5541C > T. b Representative images of the embryoid body (EB)-mediated in vitro spontaneous differentiation into three germ layers; TUJ1 (ectoderm, red), αSMA (mesoderm, red), AFP (endoderm, red). c Expression of other representative pluripotency genes in the established iPSC lines. d Silencing of transgenes expression in the established iPSC lines. Parental myoblasts after 7 days of transduction were also used as positive samples for both control and the patient. Expression level of each transgene was calculated using ΔΔCT-based relative quantification method by real-time PCR. Measurements were performed in triplicate. ND: Not Detected