Fig. 1

TsSP induce non-classical human monocytes. Primary human monocytes were cultured for 16 h in the presence or absence of TsSP (40 μg/ml) and subsequently analysed by flow cytometry for classical and non-classical monocyte markers like (a and b) CD14 and CD16 as well as (c-e) CCR2 and CX₃CR1 on live gated cells. Experiments were performed in triplicate using cells derived from 5 different human donors and the results are presented by FACS plots (a and c) or the mean percentage of positive cells +/− SEM (b, d and e). *p < 0.05 as determined by Students t test