Fig. 6
Digital neuropathological quantification using positive pixel algorithm of IBA1+ immunostaining in the hippocampus of autopsy cases. Representative example of (a) IBA1 staining and a digitally generated mark-up showing the ability of the positive pixel algorithm to detect the staining. Digital neuropathological quantification of the IBA1 staining using the positive pixel algorithm is shown for the (b) WM, (c) sub, (d) CA1 (F4,38 = 5.0943; p = 0.0025), (e) CA2/3 (F4,38 = 4.8888; p = 0.0032), (f) CA4, (g) DG,, and for the (h) average of the hippocampal formation (F4,38 = 3.0201; p = 0.0311). Circles represent an individual case, with mean and SEM shown for the group. Statistical comparisons: *p < 0.05 compared to AD cases. § p < 0.05 compared to HS-aging cases. ‡ p < 0.05 compared to AD + HS-aging cases. (i) Heatmap summarizes the results in (b-h) (also see Table 2)