Fig. 5

Digital neuropathological quantification using nuclear algorithm of number of IBA1⁺ cells in the hippocampus of autopsy cases. Representative example of (a) IBA1 staining and a digitally generated mark-up showing the ability of the nuclear algorithm to detect six stained cells. Digital neuropathological quantification of the IBA1 staining using the nuclear algorithm is shown for the (b) WM, (c) sub, (d) CA1, (e) CA2/3, (f) CA4, (g) DG, and for the (h) average of the hippocampal formation.. Circles represent an individual case, with mean and SEM shown for the group. Statistical comparisons: ^§ p < 0.05 compared to HS-aging cases. (i) Heatmap summarizes the results shown in (b-g) (also see Table 2)