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Fig. 10 | Acta Neuropathologica Communications

Fig. 10

From: Disease-related microglia heterogeneity in the hippocampus of Alzheimer’s disease, dementia with Lewy bodies, and hippocampal sclerosis of aging

Fig. 10

Disease specific patterns in IBA1+ microglia morphology. (a) Representation of microglia morphologies seen in the hippocampus of aged individuals. The number of microglia was quantified at 40x magnification in five 250 x 250 μm regions of interest (ROIs) that were randomly placed and evenly spaced in the CA1 region. Following the classification shown in (a), IBA+ microglia were classified as either (b) ramified (F4,38 = 5.3533; p = 0.0019), (c) hypertrophic (F4,38 = 5.5082; p = 0.0016), (d) dystrophic (F4,38 = 5.7249; p = 0.0012), (e) rod-shaped, or (f) amoeboid (F4,38 = 3.9836; p = 0.0093). (g) The number of microglia (F4,38 = 7.2694; p = 0.0002) in the five classifications was summed to get the total number of microglia. The gray circles in (b-g) represent the average number of microglia per mm2 for an individual case, with mean and SEM shown for each group (see also Table 2). Statistical comparisons: § p < 0.05 compared to HS-aging cases. p < 0.05 compared to AD + HS-aging cases. (h) As the total number of microglia significantly varied by group, the number of microglia in each of the five classifications was plotted as a percent of the total number of microglia to illustrate the disease-related patterns in microglia morphology (also see Table 2)

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