Fig. 3
From: Pathogenic aquaporin-4 reactive T cells are sufficient to induce mouse model of neuromyelitis optica
Histology of tissues from wildtype mice receiving unpolarized AQP4-reactive T cells (A, D, G, J) versus wildtype mice receiving Th17-polarized AQP4-reactive T cells (B, C, E, F, H, I, K, L). A. Spinal cord parenchyma stained for CD3+ T cells shows rare, scattered cell (arrow), compared to B. spinal cord sections from wildtype recipients of Th17-polarized AQP4-reactive T cells which shows intense perivascular CD3+ T cell infiltrates. C. Areas of demyelination (red) within white matter tracts (blue) are visible within inflammatory lesions. D. Longitudinal sections of optic nerves stained for CD3+ T cells shows rare, scattered cell (arrow), compared to E. optic nerve sections from wildtype recipients of Th17-polarized AQP4-reactive T cells which shows intense perivascular CD3+ T cell infiltrates (arrow) F. Areas of demyelination (arrow pointing to red) within white matter tracts (blue) are visible within inflammatory lesions. G. Brain parenchyma stained for CD3+ T cells shows rare, scattered cell (arrow), compared to H. a brain section from wildtype recipients of Th17-polarized AQP4-reactive T cells which shows intense CD3+ T cell infiltrates, such as this lesion around the 3rd ventricle (arrow). I. AQP4-reactive T cells do not appear to change AQP4 staining either in lesions or in normal appearing spinal cord, optic nerve or brain despite widespread inflammation and demyelination (representative section from spinal cord shown). J. Despite expression of AQP4 in solid organs, rare AQP4-reactive CD3+ T cells appear scattered throughout these organs both in the unpolarized and Th17-polarized wildtype recipients. Lung from unpolarized shown here with arrow pointing to CD3+ cells. K. Lung section from Th17-polarized recipient showing normal lung with occasional CD3+ cells (arrow). L. Muscle from Th17 polarized mice show no evidence of inflammation (arrow pointing to rare CD3+ T cells)