Figure 8

Preventive MB administration increases protein degradation. (a) Levels of autophagy- (beclin, HSC70), lysosome- (lamp2a), and proteasome-related (PSMD13) proteins in hippocampus lysates of age-matched untreated Tau^ΔK mice compared to MB-treated Tau^ΔK mice for 14.5mo, 6mo and 3mo. β-actin serves as loading control. (b) Quantification of (a). While both preventive MB treatment strategies (14.5mo and 6mo MB) increase levels of beclin, HSC70, Lamp2a and PSMD13, therapeutic MB treatment for 3mo leads to a partial increase of beclin and Lamp2a whereas levels of HSC70 and PSMD13 remain unaffected in comparison to untreated Tau^ΔK mice. Protein levels were normalized to β-actin. Bars indicate mean values ± SEM. Statistics: one-way analysis of variances with post-hoc Newman-Keuls multiple comparisons test. Asterisks indicate significant differences in comparison to untreated Tau^ΔK mice; *: p < 0.05; **: p < 0.01; ***: p < 0.001; n, number of samples.