Figure 4

Model system-specific microglia transcriptional signatures. a) Heatmap of model system-specific hub genes. The top 100 most significantly associated module membership-genes from the App-Ps1, Sod1, Aged, and Ercc1 blue modules. Genes belonging to the consensus network were removed. The other genes were grouped according to significant association with both Aging models (green sidebar), both Disease models (dark-pink sidebar), physiological aging (dark-green sidebar), Ercc1 (dark-olivegreen sidebar), App-Ps1 (purple sidebar), and Sod1 (orange sidebar). b) Quantitative RT-PCR validation of consensus hub genes. RNA was isolated from App-Ps1 and Ercc1 FACS sorted microglia and mRNA expression levels were determined. The fold expression, normalized to HMBS, compared to its control with the standard error is depicted. c) Quantitative RT-PCR validation of model-specific hub genes. RNA was isolated from App-Ps1 and Ercc1 FACS sorted microglia and mRNA expression levels were determined. The fold expression, normalized to HMBS, compared to its control with the standard error is depicted. d) General aging microglia are significantly enriched for KEGG ribosome and IFNa-ß signaling. GSEA was used to determine differences between consensus chronic activation and acute activation. Primed microglia are enriched for KEGGs ribosome and IFNa-ß signaling.