Figure 6

siRNA-mediated knockdown of HDAC2 induces acetylation of histone 4, caspase 3-like activity and sub-G0/G1 fraction, and reduces cell number of MYC amplified MB cells. a siRNA-mediated knockdown reduces HDAC2 protein and induces acetylation of histone 4 (H4) in MYC amplified cells MED8A and HD-MB03 72 h after transfection, as determined by western blot. HDAC2 ratio: HDAC2/beta-actin ratio, quantification of HDAC2 protein relative to untreated. Of note: The upper band in the beta-actin labeled blot of HD-MB03 cells is the signal from the HDAC2 detection, which was performed before the beta-actin detection. b-d siRNA-mediated knockdown induces caspase 3-like activity (72 h after transfection) and Sub-G0/G1 fraction (96 h after transfection), and reduces the number of viable cells (96 h after transfection) in MYC amplified cells MED8A and HD-MB03, as measured by caspase 3-like activity assay b, Nicoletti staining and flow cytometric analysis c, and trypan blue staining d, respectively. Bars depict mean and standard error of three independent experiments, significant differences are indicated: * = p < 0.05 (one way ANOVA test).