Figure 5

Class I HDAC inhibitors potently inhibit cell growth of MYC amplified medulloblastoma (MB) cells in vitro. Bars and error bars depict mean and standard error of numbers of viable cells a and cell viability (number of viable cells/number of total cells) b after 72 h of treatment, as determined by trypan blue staining. Class I selective HDAC inhibitors vorinostat (top) and MS-275 (bottom) potently inhibit cell growth of MYC amplified MB cells (MED8A and HD-MB03), while cell growth of MYC non amplified MB cells (UW228-2) is reduced only at higher concentrations. Bars depict mean and standard error of three independent experiments, significant differences between cell lines are indicated: * = p < 0.05, ** = p < 0.01, *** = p < 0.001 (unpaired t-test). ampl: amplified