Figure 3

HDAC inhibitors MAZ1863 and MAZ1866 are highly selective and potently inhibit class IIa activity. Both MAZ1863 (top) and MAZ1866 (bottom) significantly inhibit intracellular class IIa HDAC activity compared to DMSO control, as measured by class IIa HDAC specific substrate turnover in a cell-based biochemical HDAC activity assay after treatment of MED8A MB cells with the compounds at indicated concentrations for 1 h. Class I/IIb HDAC activity is not inhibited by MAZ1863 or MAZ1866, while vorinostat significantly inhibits class I/IIb HDAC activity, as expected. Bars depict mean and standard error of three independent experiments, significant differences compared to DMSO control are indicated: * = p < 0.05, ** = p < 0.01, *** = p < 0.001 (unpaired t-test).