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Figure 5 | Acta Neuropathologica Communications

Figure 5

From: PGC-1α activity in nigral dopamine neurons determines vulnerability to α-synuclein

Figure 5

PGC-1α protects primary neuronal cultures of PGC1α-KO mice against oxidative stress induced by aSyn. Seven day-old primary neuronal cultures were derived from the cerebral cortex of PGC1α-KO (a) or WT mice (b). Individual cultures were co-infected either with the non-coding and aSyn vectors (NCV + aSyn), or with the PGC-1α and aSyn vectors (PGC1α + aSyn). H2O2 concentrations were measured in cell culture media at 7 days post-infection. Note the significant increase in H2O2 production in PGC1α-KO neurons expressing aSyn, which is prevented by PGC-1α expression. In contrast, aSyn does not cause any significant increase in H2O2 production in neurons derived from WT mice. Statistical analysis: one-way ANOVA with Newman-Keuls post-hoc test; NI: n = 14; NCV + aSyn: n = 12; PGC1α + aSyn: n = 14; ***p < 0.001. Note that raw values of H2O2 production were obtained from separate experiments for neurons derived from PGC1α-KO and WT mice and are therefore not comparable.

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