Figure 2

Comparison of intraneuronal hα-syn expression after transduction with hα-syn-AAV prep 1 vs. prep 2. Analyses were performed at 6 weeks post treatment. (a, b) Representative sections of the MO (Bregma: −13.68 mm) were obtained from rats treated with (a) hα-syn-AAV prep 1 or (b) prep 2. Tissues were immunostained with anti-hα-syn and counterstained with cresyl violet. The DMnX is delineated in red. Scale bar in (b) = 100 μm. (c) Nissl-stained neurons (striped bars) and hα-syn-immunoreactive cells (solid bars) were counted in the left (injected side) DMnX of animals treated with hα-syn-AAV prep 1 (n = 7) or prep 2 (n = 7). Values (means ± SEM) are expressed as percent of the total number of Nissl-stained neurons. (d, e) Representative images of DMnX neurons immunostained with an anti-hα-syn antibody from rats treated with (d) hα-syn-AAV prep 1 or (e) prep 2. Scale bar in (e) = 20 μm. (f) Fluorescence intensity of hα-syn-immunoreactive DMnX neurons was measured by optical densitometry in rats treated with hα-syn-AAV prep 1 (n = 4) or prep 2 (n = 4). Values (pixels) are means ± SEM.