TDP-43 as a possible biomarker for frontotemporal lobar degeneration: a systematic review of existing antibodies

Table 2 Overview of existing ELISAs, with detailed information about used antibodies

	Antibody (Epitope)	Manufacturer/First report	Clonality	Species	Target detected	Times reported	References
Capture	*2E2-D3* (AA 205–222)	Abnova, Novus, Abcam, etc.	mono	mouse	• full length TDP-43	8	[41-45,48-50]
Detection	*10782* (AA 203–209 and AA near N-terminus)	ProteinTech	poly	rabbit	• (some CTFs)	8	[41-45,48-50]
Capture	*2E2-D3* (AA 205–222)	Abnova, Novus, Abcam, etc.	mono	mouse	• pTDP-43	1	[42]
Detection	*TIP-PTD-P02* (pS409/410, AA 405–414)	Cosmo Bio/Hasegawa 2008	poly	rabbit	• (some pCTFs)	1	[42]
Capture	*10782* (AA 203–209 and AA near N-terminus)	ProteinTech	poly	rabbit	• full-length TDP-43	1	[46]
Detection	60019 (AA 203–209)	ProteinTech	mono	mouse	• full-length TDP-43	1	[46]
Capture	not specified ^a	EIAab	poly	rabbit	• pTDP-43	1	[46]
Detection	SAB4200223 (pS409)^a	Sigma	poly	rabbit	• (pCTFs)	1	[46]
Capture	205 (AA 262–391)	Kwong 2014	mono	mouse	• (full length TDP-43)	1	[47]
Detection	1039C/C-t TDP-43 (AA 394–414)	Igaz 2008	poly	rabbit	• CTFs
Detection	1039C/C-t TDP-43 (AA 394–414)	Igaz 2008	poly	rabbit	• (pCTFs)

^aInformation provided by kit manufacturer, confirming published assay details. Abbreviations: CTFs, C-terminal fragments; pTDP-43, phosphorylated TDP-43; pCTFs, phosphorylated C-terminal fragments. Bold italic antibodies are those which are considered “standard” when studying TDP-43.

ISSN: 2051-5960