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Figure 3 | Acta Neuropathologica Communications

Figure 3

From: IFNβ secreted by microglia mediates clearance of myelin debris in CNS autoimmunity

Figure 3

Microglia but not astrocytes or neurons are the primary source of IFNβ production in situ . a-f Immunofluorescent analysis of organotypic cerebellar slice cultures (OSCs) for co-expression of IFNβ/YFP and different cell markers. OSCs were prepared as described in the methods and stimulated with 50 μg/ml poly (I:C) for 24 h. OSCs were stained for Iba1 (a), GFAP (b), NF-M (c), CD68 (d), Mac3 (e) and TLR3 (f) together with IFNβ/YFP. Scale bars represent 25 μm. g and h EAE was induced by immunization with 200 μg MOG35–55 peptide in IFNβmob/mob mice. Animals with a clinical score >2 were sacrificed at d17 and processed for histological analysis. Shown are representative spinal cord lesions stained for CCR2 (g) and TLR3 (h). Scale bars represent 25 μm. i Immunofluorescent analysis shows IFNβ/YFP-expressing microglia in close association with myelin debris enriched areas in OSCs. OSCs were prepared and stimulated as described above and stained for MBP and IFNβ/YFP. Scale bar represents 25 μm.

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