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Table 2 Antibodies used and antigen unmasking procedures employed

From: Loss of angiotensin II receptor expression in dopamine neurons in Parkinson’s disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related nucleic acid oxidation and caspase-3 activation

Antibody Clone/type Epitope Unmasking Dilution Source
α-synuclein 42, Mouse IgG1 Rat α-synuclein 15-123 70% Formic acid for 10 min 1:500 Becton-Dickenson
AT1 p Goat Synthetic peptide mapping @ N-terminus of human AT1 Microwave in 10 mM citrate buffer pH 6 for 30 min 1:100 Santa Cruz
Calbindin D28k p Rabbit Rat calbindin D28k Microwave in 10 mM citrate buffer pH 6 for 30 min 1:500 Swant
DNA/RNA damage m Mouse 15A3 8-hydroxy-guanosine-BSA and -casein conjugates Microwave in 10 mM citrate buffer pH 6 for 30 min 1:100 StressMarq
GFAP p Chicken Bovine full length native protein Trypsin @37°C for 10 min 1:1000 Aves Labs
Iba1 p Rabbit Synthetic peptide of C-terminus of Iba1 conserved between humans, rats, and mice Microwave in 10 mM citrate buffer pH 6 for 30 min 1:400 Wako
Nup62 m Mouse Human nucleoporin 62 aa. 24-178 Microwave in 10 mM citrate buffer pH 6 for 30 min 1:50 BD Transduction Laboratories
Pan-Neuronal m Mouse Whole neuron marker Microwave in 10 mM citrate buffer pH 6 for 30 min 1:500 Millipore
Nox4 m Rabbit Synthetic peptide within NADPH binding domain of human Nox4 (Q9NPH5) Microwave in 10 mM citrate buffer pH 6 for 30 min 1:200 Abcam
S100B Rabbit 35HT3-10 Trypsin @37°C for 10 min 1:1000 Dr. Linda Van Eldik
Tyrosine hydroxylase p Rabbit Synthetic peptide mapping @ N-terminus of human TH Microwave in 10 mM citrate buffer pH 6 for 30 min 1:1000 Pel-Freez
  1. Abbreviations: m monoclonal and p polyclonal.
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Acta Neuropathologica Communications

ISSN: 2051-5960

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