Loss of angiotensin II receptor expression in dopamine neurons in Parkinson’s disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related nucleic acid oxidation and caspase-3 activation

Table 2 Antibodies used and antigen unmasking procedures employed

Antibody	Clone/type	Epitope	Unmasking	Dilution	Source
α-synuclein	42, Mouse IgG1	Rat α-synuclein 15-123	70% Formic acid for 10 min	1:500	Becton-Dickenson
AT1	p Goat	Synthetic peptide mapping @ N-terminus of human AT1	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:100	Santa Cruz
Calbindin D28k	p Rabbit	Rat calbindin D28k	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:500	Swant
DNA/RNA damage	m Mouse 15A3	8-hydroxy-guanosine-BSA and -casein conjugates	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:100	StressMarq
GFAP	p Chicken	Bovine full length native protein	Trypsin @37°C for 10 min	1:1000	Aves Labs
Iba1	p Rabbit	Synthetic peptide of C-terminus of Iba1 conserved between humans, rats, and mice	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:400	Wako
Nup62	m Mouse	Human nucleoporin 62 aa. 24-178	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:50	BD Transduction Laboratories
Pan-Neuronal	m Mouse	Whole neuron marker	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:500	Millipore
Nox4	m Rabbit	Synthetic peptide within NADPH binding domain of human Nox4 (Q9NPH5)	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:200	Abcam
S100B	Rabbit	35HT3-10	Trypsin @37°C for 10 min	1:1000	Dr. Linda Van Eldik
Tyrosine hydroxylase	p Rabbit	Synthetic peptide mapping @ N-terminus of human TH	Microwave in 10 mM citrate buffer pH 6 for 30 min	1:1000	Pel-Freez

ISSN: 2051-5960