Figure 6

Erythrocytes and leukocytes infiltrate the brain of aged rTg4510 mice. Sections from 6-, 9-, and 12-month old rTg4510 and wild-type mice were stained with hematoxylin and eosin (H&E) and a red blood cells (RBCs) (± SEM) were manually counted throughout the brain. *** p <0.001. A 40x representative image of b H&E staining from 12-month old rTg4510 and wild-type mice is shown. Scale bar represents 50 μm; 20 μm inset. Arrowheads indicate positive RBCs. T cell lymphocytes were stained with c CD3⁺ antibody and counted (± SEM) in 6-, 9-, and 12-month old rTg4510 and age-matched wild-type mouse tissue. *** p <0.001. CD3⁺ T cells were found in 12-month old rTg4510 mice in the d hippocampus and in and around the e ventricles. Arrowheads indicate positive CD3⁺ T cells. However, there was very little positive staining was found in the f tissue or ventricles of age-matched wild-type littermates. Scale bar represents 50 μm in the hippocampus and ventricle images; inset scale bar represents 20 μm. T cell lymphocytes stained for g CD4⁺ were counted (± SEM) in 6-, 9-, and 12-month old rTg4510 and wild-type mouse tissue. *** p <0.001. CD4⁺ T cells were found in the h hippocampus and in and around the i ventricles of 12-month old rTg4510 backspace. Similar to CD3⁺ T cells, there was little j CD4⁺-positive staining in the tissue or ventricles of age-matched wild-type littermates. Arrowheads indicate CD4⁺ positive T cells. Scale bar represents 50 μm in the hippocampus and ventricle images; inset scale bar represents 20 μm.