Increase of neuronal nuclei volume in teenagers and adults with autism, and decrease in control cohort. Estimates of nucleus volume (μm3) in three age groups of subjects with autism revealed significant increase in eight brain regions (white arrows). In the control cohort, the volume of neurons decreased significantly in 14 regions. The significance level was computed by controlling for post-mortem interval, days of fixation, days of dehydration, and brain weight loss during dehydration. Large arrows indicate a significant difference in both age groups; small arrow marks a significant difference in one of two age groups.