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Figure 6 | Acta Neuropathologica Communications

Figure 6

From: LAMP-2 deficiency leads to hippocampal dysfunction but normal clearance of neuronal substrates of chaperone-mediated autophagy in a mouse model for Danon disease

Figure 6

Analysis of steady-state levels of CMA substrates in brain. (a-d) Immunoblots and respective densitometry of lysed hippocampi/cortices from wild-type (WT) and LAMP-2-deficient (LAMP-2-/y) mice showing protein levels of LAMP-2 (a), MEF2D (b), GAPDH (c) and huntingtin (Htt) (d). (e-h) Immunoblots and respective densitometry of lysates from N2a cells either non-transfected (non) or stably transfected with scramble shRNA (scmbl) or shRNA targeting LAMP-2 mRNA showing steady-state levels of LAMP-2 (e), MEF2D (f), GAPDH (g) or Htt (h) (actin was used to control loading; *denotes a non-specific band for MEF2D). LAMP-2 levels were not affected by scramble (scmbl) shRNA.

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Acta Neuropathologica Communications

ISSN: 2051-5960

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