Accumulation of p62 and filipin positive autophagic vesicles correlates with axonal and synaptic pathology in mice lacking LAMP-2. Representative histological brain sections of wild-type (WT) and LAMP-2-deficient (LAMP-2-/y) mice stained for (a) p62 using DAB (sections were costained with Nissl; arrows point to p62-positive punctae; zoomed image shown to right of main images). (b) Filipin stained sections showing cholesterol storage within the subiculum of LAMP-2-/y mice (insets show zoomed images of outlined area; arrows highlight storage). (c) High power electron micrographs from the subiculum and hippocampal CA3 region from LAMP-2-/y mice. Neurites and presynaptic terminals showed numerous autophagosomes with storage material of different nature (arrows point to lipofuscin (L), autophagosomes (AP), autophagic vacuoles (AV) and lipid whorls (LW), S denotes a synaptic spine).