Figure 2

LAMP-2 expression in brain. LAMP-2 (green) vesicular staining detected in (a) MAP-2 (red) positive cultured primary neurons and (b) GFAP (red) positive primary astrocytes (LAMP-2-deficient (LAMP-2^-/y) cultures were used to control for antibody specificity). (c) Immunoblot and respective densitometric analysis of LAMP-2 (n = 5) showing its expression throughout murine brain (cortex from a LAMP-2^-/y mouse was used to show specificity of the LAMP-2 antibody; actin was used as a loading control; *p < 0.05, **p < 0.01, ***p < 0.001). Quantitative RT-PCR of isoforms (d) LAMP-2A and (e) LAMP-2B in respective brain regions. (f) Representative histological brain sections stained for LAMP-2 using the DAB method and counterstained with Nissl. No signal was detected in LAMP-2^-/y brains. Enriched expression was observed in the pyramidal layer of the hippocampus, within the Purkinje cell layer of the cerebellum and within a region of the pons. Insets show zoomed images of regions outlined.