Figure 5

TTR is internalized by satellite cells from the myenteric plexus. A) TTR immunohistochemical staining on colon myenteric plexus from WT, TTR KO and Hsf/V30M animals. Scale bar 20 μm. B) Double immunofluorescence between TTR (green) and S100 (red), a satellite cell marker, showing TTR inside these cells. Scale bar 14 μm; higher magnification 5 μm. C) Representative confocal image of myenteric plexus section showing a satellite cell with intracellular TTR staining (green) colocalizing with EEA1 (red) (arrows). Scale bar 4 μm. D) Confocal microscopy image with anti-TTR antibody (green) and lysosome marker (Lamp1; red). Nuclei stained blue with DAPI. Arrows indicate colocalization of TTR with lysosomes. Scale bar 5 μm.