Figure 4

Representative photomicrographs of the lumbar anterior horn (a-l) and the hypoglossal nucleus (m). Intracytoplasmic inclusion bodies immunoreactive for TDP-43 are identifiable in the lumbar AHCs (a, arrows). A glial cytoplasmic inclusion (GCI) is evident with the anti-TDP-43 antibody (b, arrow). Analyses of consecutive sections indicated that TDP-43-positive inclusions in the AHCs (c, e, g, i, arrows) are also positive for ubiquitin (Ub; d, arrow), for p62 (f, arrow), and for OPTN (h, arrow), but devoid of immunoreactivity for VCP (j, arrow). The nucleus of the inclusion-bearing neuron lacks immunolabeling for TDP-43 (c, e arrowhead). Fragmentation of the Golgi apparatus is apparent in the AHCs immunostained with TGN-46, a marker protein of the Golgi, in comparison with the preserved Golgi apparatus in the non-motor neuron of the posterior horn (l, arrow). A Bunina body in the hypoglossal nucleus is immunopositive for cystatin C (m). Scale bars = 25 μm (a, c-m), 12.5 μm (b).