Transcript profiling of different types of multiple sclerosis lesions yields FGF1 as a promoter of remyelination

Mohan, Hema; Friese, Anita; Albrecht, Stefanie; Krumbholz, Markus; Elliott, Christina L; Arthur, Ariel; Menon, Ramesh; Farina, Cinthia; Junker, Andreas; Stadelmann, Christine; Barnett, Susan C; Huitinga, Inge; Wekerle, Hartmut; Hohlfeld, Reinhard; Lassmann, Hans; Kuhlmann, Tanja; Linington, Chris; Meinl, Edgar

doi:10.1186/s40478-014-0168-9

Acta Neuropathologica Communications

Table 2 Expression levels of genes potentially regulating oligodendrocytes and their fold changes in demyelinated inactive versus remyelinated lesions

From: Transcript profiling of different types of multiple sclerosis lesions yields FGF1 as a promoter of remyelination

Gene name	CWM		Remyelinated		Demyelinated		Active		*Re/CWMl*		*Re/De*
	mean	SEM	mean	SEM	mean	SEM	mean	SEM	*ratio*	p	*ratio*	p
PDGFs
PDGFA	19.38	2.59	31.78	10.91	15.45	4.47	51.82	34.22	1.64	0.61	2.06	0.26
PDGFB	1.23	0.28	1.71	0.30	6.79	2.91	31.65	29.42	1.39	0.26	0.25	0.48
PDGFC	0.52	0.09	1.45	0.47	1.19	0.48	2.56	1.69	2.78	0.038	1.22	0.61
PDGFD	0.14	0.05	0.37	0.22	0.83	0.46	0.08	0.03	2.61	1.00	0.45	0.45
FGFs
FGF1	39.42	2.61	85.40	11.54	48.15	19.53	58.18	16.80	2.17	0.010	1.77	0.11
FGF2	3.82	0.56	9.23	0.88	10.25	2.62	6.08	0.63	2.41	0.010	0.90	0.91
FGF5	0.01	0.01	0.00	0.00	0.01	0.01	0.00	0.00	0.00*	0.15	0.00*	0.15
FGF8	0.00	0.00	0.06	0.05	0.02	0.01	0.01	0.00	16.25*	0.74	2.69*	0.72
FGF9	0.04	0.02	0.02	0.01	0.87	0.53	0.01	0.00	0.44*	0.71	0.02*	0.066
Semaphorins
SEMA3A	0.58	0.11	0.77	0.10	1.77	0.75	0.71	0.25	1.32	0.26	0.43	0.35
SEMA3B	6.33	1.73	10.48	4.21	2.82	1.50	5.37	3.57	1.66	0.61	3.72	0.11
SEMA3C	4.84	1.06	7.13	0.75	2.10	0.59	6.13	1.73	1.47	0.17	3.40	*0.010*
SEMA3D	0.55	0.09	2.18	0.57	1.08	0.37	0.85	0.23	3.94	0.019	2.02	0.11
SEMA3E	0.41	0.04	0.91	0.29	0.49	0.15	0.69	0.23	2.21	0.26	1.84	0.17
SEMA3F	0.02	0.01	0.02	0.01	0.20	0.10	1.91	1.89	0.93*	0.91	0.10*	0.44
SEMA3G	0.23	0.05	0.16	0.06	0.54	0.31	1.18	0.98	0.68	0.48	0.30	0.91
SEMA4A	0.22	0.06	0.21	0.05	1.00	0.36	0.29	0.04	0.95	0.91	0.21	0.067
SEMA4B	2.63	0.31	8.90	2.16	8.46	3.46	18.31	14.74	3.39	0.010	1.05	0.48
SEMA4C	4.49	0.77	8.07	2.81	3.67	0.83	9.90	6.17	1.80	0.26	2.20	0.11
SEMA4D	16.61	3.51	24.36	7.25	4.72	2.38	23.80	11.36	1.47	0.61	5.16	*0.019*
SEMA4F	0.40	0.04	1.27	0.13	3.09	0.67	4.83	4.19	3.18	0.010	0.41	0.11
SEMA4G	0.35	0.07	0.44	0.13	0.48	0.28	0.89	0.43	1.26	0.61	0.91	0.59
SEMA5A	2.38	0.56	3.06	0.45	3.08	1.07	8.98	7.60	1.28	0.61	0.99	0.91
SEMA5B	0.45	0.09	0.78	0.18	2.34	0.52	1.32	0.53	1.72	0.17	0.33	0.11
SEMA6A	28.24	6.78	45.65	12.14	12.75	3.45	31.24	4.13	1.62	0.26	3.58	*0.010*
SEMA6B	0.73	0.18	1.95	0.78	9.90	4.74	31.22	30.80	2.67	0.07	0.20	0.17
SEMA6C	0.05	0.01	0.07	0.03	0.13	0.06	0.06	0.02	1.53	0.28	0.54	0.83
SEMA6D	7.30	1.55	13.26	2.93	4.43	0.84	5.70	1.43	1.82	0.11	3.00	*0.019*
SEMA7A	2.85	0.75	3.91	0.35	0.93	0.50	2.99	1.56	1.37	0.48	4.22	*0.019*
Chemokines
CXCL1	0.18	0.07	1.18	0.32	4.46	3.25	1.30	1.04	6.51	0.019	0.27	0.76
CXCL2	0.17	0.08	0.62	0.14	1.10	0.47	3.49	2.96	3.70	0.019	0.56	0.91
CXCL8	0.23	0.06	0.51	0.19	0.71	0.15	0.52	0.42	2.22	0.26	0.71	0.61
CXCL10	0.02	0.01	0.05	0.02	0.04	0.03	0.88	0.47	1.94*	0.76	1.09	0.66
CXCL12	2.51	0.37	3.65	0.70	30.09	23.06	24.58	16.09	1.45	0.35	0.12	0.76
IL6 family
CNTF	0.19	0.04	0.52	0.11	0.20	0.11	0.69	0.50	2.66	0.019	2.65*	0.069
CLCF1	0.02	0.01	0.16	0.13	0.48	0.23	0.55	0.34	6.62*	0.35	0.34	0.83
CTF1	0.17	0.03	0.44	0.13	0.82	0.29	1.66	1.36	2.59	0.17	0.54	0.61
IL6	0.06	0.02	0.07	0.03	0.38	0.31	0.06	0.02	1.27	0.76	0.19	0.83
IL11	0.01	0.00	0.07	0.05	0.04	0.03	0.02	0.01	5.11*	1.00	1.63	0.91
LIF	0.01	0.00	0.03	0.02	0.76	0.70	0.43	0.37	5.17*	0.40	0.04	1
IGFs
IGF1	0.35	0.10	0.32	0.12	0.63	0.23	1.46	0.98	0.94	1.00	0.51	0.61
IGF2	0.23	0.01	0.33	0.12	6.04	4.62	1.95	1.23	1.42	0.26	0.06	0.39
Miscellaneous
CTGF	1.70	0.50	4.53	0.90	13.40	4.85	9.77	7.80	2.67	0.038	0.34	0.26
FIGF	0.25	0.07	0.28	0.07	0.60	0.51	0.29	0.10	1.09	0.76	0.46	0.34
HGF	0.12	0.02	0.37	0.12	0.31	0.14	0.99	0.40	3.05	0.26	1.17	0.45
LINGO1	0.15	0.11	0.77	0.30	1.42	0.50	0.40	0.14	5.18	0.06	0.54	0.61

CWM: Control white matter, Remyelinated: Remyelinated lesion, Demyelinated: Demyelinated inactive lesion, Active: Demyelinated active lesion, Re/CWM and De/Re ratio: remyelinated vs. control white matter and vs. demyelinated inactive lesions, respectively; p value were calculated by 2-sided U tests, unadjusted for multiple testing. p-values < 0.05 are printed in bold. 0.00: Values were below our detection limit, which was 0.01% GAPDH. Specimens analyzed: 6 normal white matter specimens from 4 subjects, 6 demyelinated inactive lesions from 4 subjects, 4 demyelinated active lesions from 3 subjects, and 4 remyelinated lesions from 3 subjects were dissected and used for qPCR analysis. The mean expression values are given as % GAPDH; SEM denotes standard error of the mean. * Ratios may be imprecise since at least one value was below or close to the detection limit.

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