dynamics in the CNS of T
-depleted mice. Intravital two-photon imaging was performed in the spinal cord meninges of T-Red x DEREG mice two days after DTx or PBS treatment. (A) Trajectories (white lines) of dsRedII+ Teff overlaid with snapshots from representative videos. One representative out of three independent experiments per treatment condition is shown (red: Teff, green: APC, scale bar: 30 μm) (B) Superimposed trajectories of Teff movements after DTx or PBS treatment. Time points with contacts with APC are indicated in red or blue, respectively. One representative out of three independent experiments per treatment condition is shown. (C) Average velocity, (D) linearity index (sum of the total displacement divided by the path length of a cell), (E) stationary phase (part of the track with velocity <3 μm/min) (± SEM) of Teff after DTx or PBS treatment (pooled data from three independent experiments (**P < 0.01, t-test) (pooled data from three independent experiments).