Figure 2

Colocalization of FMRpolyG (8FM) and ubiquitin in intranuclear inclusions in hippocampus, cerebellum and of non-CNS tissues of a FXTAS patient. Staining for ubiquitin (green), FMRpolyG (8FM; red) and DAPI (blue). Colocalization of ubiquitin and FMRpolyG (yellow) is seen in a hippocampus, b cerebellum, c kidney, d adrenal gland, e cardiomyocytes, and f thyroid; g quantification of inclusions containing ubiquitin and/or FMRpolyG (n = 100). Scale bars represent 10 μm.