Figure 5
From: Decreased EAAT2 protein expression in the essential tremor cerebellar cortex
EAATs in astrocytic processes surrounding PC axonal initial segment (AIS) in the ET cerebellar cortex. Dual immunofluorescence with anti-EAAT1 or anti-EAAT2 (Alexa 488, green) antibody and anti-Lingo-1 (Alexa 594, red) antibody in the region adjacent to PC AIS (A-F). Basket cell pinceau were visualized with anti-Lingo-1 antibody. We found that only very low levels of EAAT1 were found in this region (A-C, arrows). In contrast, EAAT2 positive astrocytic processes interdigitated with Lingo-1 positive basket cell processes, surrounding PC AIS (D-F, arrows). Dual immunofluorescence with anti-EAAT2 (Alexa 488, green) and anti-Calbindin (Alexa 594, red) antibody to investigate the relationship between PC axonal torpedoes and EAAT2-expressing astrocytic processes (G-I). EAAT2-expressing astrocytic processes surrounded a PC axonal torpedo (Arrowheads, H-I). Immunohistochemistry with anti-EAAT2 antibody also revealed EAAT2-expressing astrocytic processes distributed around a PC axonal torpedo (Arrowheads, J). p: Purkinje cell body, t: PC axonal torpedo. Scale bar 25 μm.