Figure 4
From: Decreased EAAT2 protein expression in the essential tremor cerebellar cortex
EAATs colocalized with astrocytic markers. Dual immunofluorescence labeling of ET cerebellar cortex with anti-EAAT1 or anti-EAAT2 (Alexa 488, green, A, D, G, J, M, P), anti-GFAP (Alexa 594, red, B, E, K, Q), and anti-glutamate synthetase (Alexa 594, red, E, N). Both EAAT1 and EAAT2 partially colocalized with GFAP or glutamine synthetase in the cerebellar cortex (C, F, L, O). The cell bodies of Bergmann glia showed stronger colocalization with EAAT1 than with EAAT2 (arrows, in D-F). Higher magnification images showed both EAAT1 and EAAT2 form puncta along the GFAP-positive astrocytic processes (G-I, P-R). p: PC body. Scale bar 25 μm.