Figure 3

EAAT expression in the cerebellar cortex. Immunohistochemistry with anti-EAAT1 or anti-EAAT2 antibody in the cerebellar cortex. EAAT1 was expressed predominantly in the Bergmann glia radial processes in the molecular layer (A, B). EAAT2 was expressed in the glia radial processes in the molecular layer and in the bushy astrocytes in the granular cell layer (C, D). Purkinje cell and granule cell bodies did not express EAATs (A-D). EAAT2 was also enriched in the astrocytic processes surrounding the PC axonal initial segment (D, arrows) m: molecular layer, g: granule cell layer, p: PC body. Scale bar 25 μm.