Figure 8

VPSPr PrP ^Sc can seed conversion in RT-QuIC. The reactions were seeded with three 10-fold dilutions of VPSPr or sCJD VV2 brain amount, normalised according the titre of PrP^res (units shown are grams PrP^res per 100 μl reaction volume). As a control, equivalent dilutions of non-CJD brain homogenate (shown as% [w/v]) were used to seed parallel reactions. Mean ThT fluorescence values (relative fluorescence units) for the quadruplicate reactions were plotted against time (hours).