Table 1 Antibodies and blocking reagents for Western blot detection of aggregated human α-synuclein, amyloid-β and tau
From: Decontamination of medical devices from pathological amyloid-β-, tau- and α-synuclein aggregates
1stAntibody | Provider and product code | Epitope | Dilution | Blocking solution | 2ndAntibody | Dilution |
|---|---|---|---|---|---|---|
LB 509 (monoclonal) | Abcam, Cambridge, UK ab27766 | aa 115-122 of α-synuclein | 1:5,000 | 0.03% (w/v) Casein in TBS with 0.05% (w/v) Tween 20 | Alkaline-phosphatase-conjugated goat anti-mouse IgG (DAKO) | 1:5,000 |
Phospho-S129 (monoclonal) | Abcam, Cambridge, UK ab51253 | α-synuclein phosphorylated at Ser129 | 1:2,000 | 3% (w/v) Low-fat milk powder in TBS with 0.05% (w/v) Tween 20 | Alkaline-phosphatase-conjugated goat anti-rabbit IgG (DAKO) | 1:10,000 |
Anti-tau (monoclonal) | Merck Millipore, Darmstadt, Germany MAB2239 | C-terminal region of tau | 1:500 | 3% (w/v) Low-fat milk powder in TBS with 0.05% (w/v) Tween 20 | Alkaline-phosphatase-conjugated goat anti-mouse IgG (DAKO) | 1:10,000 |
AT8 (monoclonal) | Thermo Scientific, Rockford, USA MN1020 | Phospho-PHF-tau (Ser202/Thr205) | 1:500 | 0.03% (w/v) Casein in TBS with 0.05% (w/v) Tween 20 | Alkaline-phosphatase-conjugated goat anti-mouse IgG (DAKO) | 1:10,000 |
6E10 (monoclonal) | Covance, Emeryville, USA SIG-39320 | aa 3-8 of Aβ | 1:2,000 | 5% (w/v) Low-fat choco powder in TBS with 0.05% (w/v) Tween 20a | Alkaline-phosphatase-conjugated goat anti-mouse IgG (DAKO) | 1:10,000 |
4G8 (monoclonal) | Covance, Emeryville, USA SIG-39220 | aa 17-24 of Aβ | 1:1,000 | 5% (w/v) Low-fat choco powder in TBS with 0.05% (w/v) Tween 20a | Alkaline-phosphatase-conjugated goat anti-mouse IgG (DAKO) | 1:10,000 |