Analysis of α-synuclein expression in MSA (n = 8) and control (n = 10) white matter. (A) mRNA expression of α-synuclein as measured by qPCR; data represent mean and SE as error bars, *p < 0.05. (B) Soluble cytosolic α-synuclein protein as measured by western blotting of Control 1–4 and MSA 1–4. β-actin was used as a loading control. (C) Relative optical density of α-synuclein-immunoreactive protein bands as quantified using ImageJ software.