| |
Apical dendrites
|
Basal dendrites
|
|---|
|
Spines per 10 μm
|
Stubby
|
Mushroom-like
|
Thin
|
Stubby
|
Mushroom-like
|
Thin
|
|---|
|
WT Sal
|
0.90±0.12
|
10.94±0.45
|
0.27±0.08
|
0.84±0.09
|
10.30±0.65
|
0.03±0.02
|
|
WT Nic
|
0.79±0.17
|
11.83±0.65ω
|
0.08±0.06
|
0.86±0.12
|
10.10±0.58
|
0.00±0.00
|
|
Tg Sal
|
1.50±0.23σ
|
12.20±0.39σ
|
0.23±0.10
|
1.16±0.11σ
|
10.01±0.62
|
0.01±0.01
|
|
Tg Nic
|
0.96±0.15σ
|
13.51±0.70σ,ω
|
0.11±0.06
|
1.40±0.13σ
|
9.74±0.61
|
0.00±0.00
|
- Number of spines per 10 μm of dendrite length, in apical and basal dendrites of CA1 pyramidal neurons from Thy1-YFP- wild type (WT) and Thy1-YFP- TgCHRNA5/A3/B4 (Tg). Mice were implanted with osmotic minipumps delivering either saline (Sal) or nicotine (Nic, 3.25 mg/Kg/d) for 7 d (n = 5-10 cells/animal; 4-5 animals/group from ≥ 3 experiments). Two-way ANOVA genotype effect σ
p ≤ 0.05, treatment effect ω
p ≤ 0.05.
