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Figure 9 | Acta Neuropathologica Communications

Figure 9

From: Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions

Figure 9

The T1-LIR 32 kDa (transduced HEK293T and P1 brain) and 50 kDa (P1 brain) species are enriched by oligonucleotide DNA affinity chromatography. (a) Total cellular extracts from HEK293T cells transduced with Ad-mThap1 were subjected to DNA affinity chromatography using a THABS oligonucleotide polymer. The 32 kDa T1-LIR species (arrow) was enriched in the eluate relative to input. (b) P1 forebrain nuclear (nuc) extract was subjected to DNA affinity chromatography using a THABS DNA oligonucleotide column. The endogenous 32 kDa (*) and 50 kDa doublet (** and arrow) T1-LIR species were enriched in the eluate relative to input. Bottom panels are the membranes stained with Ponceau S.

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