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Figure 3 | Acta Neuropathologica Communications

Figure 3

From: Dystonia type 6 gene product Thap1: identification of a 50 kDa DNA-binding species in neuronal nuclear fractions

Figure 3

Levels of Thap1 T1-LIR 32, 50, and 75 kDa species are dramatically reduced in the nuclear compartment of Thap1 -null embryos. (a) RT-qPCR was performed on mRNA derived from whole embyos, E9 Thap1 WT, heterozygote null and homozygote null. mTHAP1 levels were normalized to TBP, and WT was arbitrarily set at 100%. (b) Immunoblots of cytoplasmic (cyt, 20 µg) and nuclear (nuc, 10 µg) extracts from E9-E12 Thap1 +/+(WT), Thap1 +/– (HET) and Thap1 –/– (KO) embryos following separation into “head” and “body” were performed using the Proteintech antibody. Only the 29 kDa T1-LIR species was observed in the cytoplasmic compartment from E9 Thap1 +/+ and Thap1 +/– body, whereas the 29 kDa, 50 kDa, and 75 kDa species were observed in the nuclear compartment from these samples (left panel). The level of the 29 kDa species in the cytoplasm was not reduced in the null mutant (middle panel), but the levels of the 29 kDa species (*), the 50 kDa doublet (** and arrow), and 75 kDa (arrowhead) species were markedly reduced in the nuclear compartment of the null embryo “head”, with a greater relative reduction in the higher Mr species than in the 29 kDa species (*) (right panel) (representative of 3 null embryos).

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