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Table 3 Measurements of brain-specific serum antibodies by ELISA

From: Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood

Patient no. Directex vivoELISPOT response [spot number] OD SD OD SD OD brain antigen/OD total IgG
Brain antigen* Brain antigen Total IgG Total IgG
1 26.0 ± 4.2 1.593 0.136 2.748 0.04 0.58
2 9.0 ± 6.4 0.974 0.094 2.609 0.084 0.373
3 86.5 ± 41.7 0.852 0.107 2.693 0.049 0.316
4 13.0 ± 2.8 1.231 0.084 2.558 0.132 0.48
10 0.0 ± 0.0 0.468 0.014 2.92 0.086 0.16
11 0.0 ± 0.0 0.437 0.034 2.636 0.024 0.166
12 0.0 ± 0.0 0.108 0.000 2.673 0.024 0.04
16 0.0 ± 0.0 0.235 0.057 2.608 0.042 0.09
17 0.0 ± 0.0 0.576 0.013 2.724 0.032 0.211
18 0.0 ± 0.0 0.147 0.034 2.837 0.112 0.052
20 0.0 ± 0.0 0.369 0.047 2.729 0.12 0.135
22 0.0 ± 0.0 0.658 0.060 2.783 0.033 0.236
  1. ELISA = enzyme-linked immunosorbent assay; ELISPOT = enzyme-linked immunospot technique; OD = optical density; SD = standard deviation.
  2. *All samples were tested in duplicate wells and are represented as mean medium-subtracted values. The cut-off value for a positive response was calculated from the means of a group of 69 healthy control donors + 5 SD (OD brain antigen/OD total IgG > 0.307).