Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood

Table 3 Measurements of brain-specific serum antibodies by ELISA

Patient no.	Directex vivoELISPOT response [spot number]	OD	SD	OD	SD	OD brain antigen/OD total IgG
Patient no.	Directex vivoELISPOT response [spot number]	Brain antigen*	Brain antigen	Total IgG	Total IgG	OD brain antigen/OD total IgG
1	26.0 ± 4.2	1.593	0.136	2.748	0.04	0.58
2	9.0 ± 6.4	0.974	0.094	2.609	0.084	0.373
3	86.5 ± 41.7	0.852	0.107	2.693	0.049	0.316
4	13.0 ± 2.8	1.231	0.084	2.558	0.132	0.48
10	0.0 ± 0.0	0.468	0.014	2.92	0.086	0.16
11	0.0 ± 0.0	0.437	0.034	2.636	0.024	0.166
12	0.0 ± 0.0	0.108	0.000	2.673	0.024	0.04
16	0.0 ± 0.0	0.235	0.057	2.608	0.042	0.09
17	0.0 ± 0.0	0.576	0.013	2.724	0.032	0.211
18	0.0 ± 0.0	0.147	0.034	2.837	0.112	0.052
20	0.0 ± 0.0	0.369	0.047	2.729	0.12	0.135
22	0.0 ± 0.0	0.658	0.060	2.783	0.033	0.236

ELISA = enzyme-linked immunosorbent assay; ELISPOT = enzyme-linked immunospot technique; OD = optical density; SD = standard deviation.
*All samples were tested in duplicate wells and are represented as mean medium-subtracted values. The cut-off value for a positive response was calculated from the means of a group of 69 healthy control donors + 5 SD (OD brain antigen/OD total IgG > 0.307).

ISSN: 2051-5960