Table 2 Primary antibodies used in the present study
Antibody | Host | Dilution | Source |
|---|---|---|---|
TPPP (C-ps B) | rabbit | 1:500 (IHC, IF) | our laboratory |
1:20,000 (WB) | |||
1:20 (IEM) | |||
TPPP (N-mab#2A8G3) | rat | 1:50 (IHC, IF) | our laboratory |
1:2000 (WB) | |||
1:20 (IEM) | |||
GFAP | mouse | 1:500 (IF) | Sigma |
IBA1 | rabbit | 1:500 (IF) | Proteintech |
MBP | rabbit | 1:500 (IF) | DAKO |
OLIG2 | rabbit | 1µg/ml (IF) | IBL |
actin | rabbit | 1:20,000 (WB) | Sigma |
lamin B1 | rabbit | 1:500 (IF) | Santa Cruz |
β-tubulin | mouse | 1:5000 (WB) | BD Pharmingen |
TOM20 | rabbit | 1:100 (IF) | Santa Cruz |
1:3000 (WB) | |||
calnexin | rabbit | 1:20,000 | Proteintech |
histone H3 | rabbit | 1:2000 (WB) | Cell Signaling |
cytochrome C | mouse | 1:200 (IF) | BD Pharmingen |
DRP1 | rabbit | 1:200 (IHC, IF) | Abcam |
MFN2 | rabbit | 1:100 (IHC) | Proteintech |
pα-syn | mouse | 1:4000 (IHC,IF) | WAKO |