Figure 5

TPPP relocalization is specific to MSA. a: Normal staining of TPPP (TPPP-C-psB) of the frontal lobe of a patient with ALS(Case 27). b-d: A biopsy specimen with oligodendroglioma (Case29). Tumor mass (* in b and c) is devoid of TPPP-immunoreactivity whereas the reaction is seen in the nearby normal region (# in b and d) (antibody: TPPP-C-psB). e-i: A parietal lobe sample from a patient with MS (Case 31). Two corresponding panels from Klűver-Barrera staining (e) and an immunohistochemistry for TPPP (TPPP-C-psB) (f) suggest reduced TPPP-immunoreactivity correlates with myelin loss. Relatively less affected area (¶ in e, f and g) shows almost normal TPPP pattern (g). In an area with severe demyelination († in e, f and h), TPPP was nearly completely lost (h). In a different area with a milder demyelination († in e, f and i), some oligodendroglia showed TPPP-immunoreactivity in their nuclei (i). a, b-d, f-i: TPPP-C-psB. Scale bar = 50 µm (a-i), 5 mm (b, e, f).