Pax3 enhances PDGF-B-induced Brainstem Gliomagenesis. a Kaplain-Meier survival curve of Ntv-a mice injected with DF1 cells expressing RCAS-Pax3, RCAS-PDGF-B, or RCAS-PDGF-B + RCAS-Pax3. b Mice from (a) were sacrificed at the onset of tumor symptoms or at 12 weeks in the absence of symptoms. Brains were harvested, fixed in formalin, and analyzed for the presence of tumors using hematoxylin and eosin (H&E) staining, and the tumors graded as described in Materials and Methods. Shown is the percentage of mice in each group with no tumor, grade II glioma, and grade III glioma. c-d Representative H&E staining (c) and IHC for proliferating cell nuclear antigen, PCNA (d) of normal brainstem (top row), PDGF-B-induced grade II BSG (middle row), and PDGF-B + Pax3-induced grade III BSG (bottom row). c Magnification is 10x in the left panels and 40x in the right panels. White arrow in lower-left panel indicates microvascular proliferation, which is enlarged in the 40x panel to its right. Scale bar for 10x is 100 μM, 40x is 25 μM. d 40x magnification; Scale bar is 25 μM.