Pax3 is expressed in mouse Brainstem Glioma. a Log expression level of Pax3 for one probe from the expression array in Figure 1. b qRT-PCR for Pax3 in normal brainstem (NBS) and brainstem glioma (BSG) tissue, n = 3 for each. Relative Pax3 expression is normalized to Actin. p = 0.01, unpaired t-test. c Log expression level of Pax3 for one of three probes from the Olig2-eGFP-L10a glioma expression array from Additional file 4: Figure S1b. d qRT-PCR for Pax3 in unsorted (Uns), GFP+, and GFP- fractions of Olig2-eGFP-L10a BSG (n = 5). Pax3 expression levels are normalized to Actin and represented as a percentage of unsorted cells. p = 0.04 Uns vs. GFP+, p = 0.002 GFP+ vs. GFP-, paired t-test. e Western blot for PAX3 (53 kDa) in the following mouse tissues from left to right: P3 cerebellum (CB), P3 cerebral cortex (NC), and P3 brainstem (NBS); adult normal cerebral cortex (NC), Cerebral Cortex Glioma (CG), adult normal brainstem (NBS), and Brainstem Glioma (BSG). ACTIN (43 kDa) is shown as a loading control. f Western blot for PAX3 (53 kDa) in PDGF-B;H3.3-K27M;p53-deficient Cerebral Cortex Glioma (CG) and Brainstem Glioma (BSG). ACTIN (43 kDa) is shown as a loading control.