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Figure 2 | Acta Neuropathologica Communications

Figure 2

From: Behavioral deficits, early gliosis, dysmyelination and synaptic dysfunction in a mouse model of mucolipidosis IV

Figure 2

Reactive astrocytosis and activation of microglia in Mcoln1 −/− mice. Representative images of GFAP immunostaning in wild-type (WT) and Mcoln1 −/− (KO) littermates at two, three and seven months of age showing increased immunoreactivity in KO in thalamus (A) or somatosensory barrelfield cortex (B). Scale bar is equal to 500 μm in low magnification images, 50 μm in higher magnification images of thalamic area and 150 μm in the images of cortex. Black selection area shows an example of VPL/VPM area of thalamus used for thresholding analysis, represented in (C). (C). Thresholding analysis of GFAP staining intensity performed for VPL-VPM region of thalamus and S1BF cortex in wild-type (WT) and Mcoln1 −/− (KO) littermates at two (n = 6 per genotype), three (n (WT) = 3; n (KO) = 4) and seven months of age (n = 4 per genotype). Data presented as mean values ± SEM. Two-way ANOVA revealed significant effect of age and genotype. Post-hoc t-test with Bonferroni correction: * - p < 0.05; *** - p < 0.001. (D), (E). Representative images of CD68 immunostaning in wild-type (WT) and Mcoln1 −/− (KO) littermates at two, three and seven months of age showing increased immunoreactivity in KO in thalamus (D) or somatosensory barrelfield cortex (E). Scale bars as in (B). Black selection area shows an example of VPL-VPM area of thalamus used for thresholding analysis, represented in (F). (F). Thresholding analysis of CD68 staining intensity in VPL/VPM region of thalamus and S1BF cortex in wild-type (WT) and Mcoln1 −/− (KO) littermates at two (n = 6 per genotype), three (n (WT) = 3; n (KO) = 4) and seven months of age (n = 4 per genotype group). Data presented as mean values ± SEM and analyzed by two-way ANOVA and post-hoc t-test with Bonferroni correction: *** - p < 0.001.

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