Figure 8

Histological evaluation of pathological tau. Representative images of AT8 and PHF1 immunostaining within the hippocampus depicted differences between CpG ODN-treated and vehicle-treated 3xTg-AD mice in both study groups ( A - D ), ( H - K ). Semiquantitative analysis of hippocampal neurons revealed reductions in AT8 (**p = 0.028) ( E ), PHF1 (*p = 0.049) ( F ) and MCI (*p = 0.036) ( G ) immunoreactivity in treated mice compared with control mice in the 7–20 month study group. Likewise, significant reductions were observed in AT8 (**p = 0.0075) ( L ) and PHF1 (*p = 0.03) ( M ) immunoreactive tau in CpG ODN-treated animals in the 11–18 month study group. There was a trend for reduced MCI immunoreactivity in treated animals from 11 to 18 months (p = 0.06) ( N ). Scale bars, 100 µm.