Figure 4

Fibrillar amyloid burden. Thioflavine-S staining revealed significant differences in hippocampal fibrillar amyloid burden between CpG ODN-treated and vehicle-treated 3xTg-AD mice ( A - D ). Thioflavine-S staining of amyloid depositions was minimal in the cortex. There was a 58% reduction (***p = 0.0004) in hippocampal fibrillar amyloid burden in the 7–20 month study group ( E ) and a 57% reduction (***p = 0.0004) in hippocampal fibrillar amyloid burden in the 11–18 month study group ( F ) as quantified using an unbiased random sampling scheme and semi- automated image analysis system. Scale bar, 100 µm.